Users can query their sequences on the DNA barcoding database for traditional medicines (http://www.tcmbarcode.cn) to easily determine the species identity of the query sequences. The procedures only require pasting the sequences queried into the text box of ITS2 or psbA–trnH and pressing the “submit” button. The results are displayed, and the best-hit sequence obtained is the closest match to the query.
Here, we take an unknown sequence as an example:
1) Open “species identification system for Traditional Chinese medicine (“http://www.tcmbarcode.cn”)
2) Click the “identification” module
3) Select corresponding maker type and paste your query sequence in query box.
4) Click “submit” button
5) In a few moments, the result page will be displayed below the query box.